Taxonomic and genetic study of a mixed oak stand

Cseke Klára; Bordács Sándor; Borovics Attila

Bulletin of Forestry Science / Volume 1 / Issue 1 / Pages 95-105
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Klára Cseke, Sándor Bordács & Attila Borovics

Correspondence

Correspondence: Cseke Klára

Postal address: H-9600 Sárvár, Várkerület 30/A.

e-mail: csekekl[at]ertisarvar.hu

Abstract

A genetic study was performed in order to analyse the taxonomic and genetic composition of a mixed oak stand rich in different oak species (Quercus spp.). Microsatellite DNS markers were applied which are suitable for identification purpose. All the adult trees were sampled on the study plot and their taxonomic classification was accomplished by numerical taxonomy. Moreover two juvenile clumps were sampled with the aim of comparing the genetic pattern of the adult and juvenile generations. Based on the results of the genetic analysis, 9% of the adult trees had clonal origin referring to the long-term coppice management on the stand. Furthermore, clones were also discovered among the individuals of the juvenile clumps indicating vegetative propagation by root shoots. The genetic structure of the adult and juvenile groups were similar with high diversity indices. The two juvenile clumps showed different affinity with the pedunculate oak and pubescence oak species respectively based on the comparison of genetic patterns.

Keywords: Quercus spp., DNA microsatellite markers, genetic diversity, coppice, juvenile clump

References22

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Bakker, E.G.; Van Dam, B.C.; Van Eck, H.J. and Jacobsen, E. 2001: The description of clones in ancient woodland of Quercus robur L. and Q. petraea (Matt.) Liebl. with microsatellites and AFLP. in: Bakker E.G. (edit.) 2001: Towards molecular tools for management of oak forests. Alterra Green World Research. Wageningen. 34–43.

Borovics A. 2000: Keresztezési kísérletek és taxonómiai vizsgálatok az őshonos tölgyek hazai alakkörében. PhD értekezés (NYME EMK).

Copini P.; Buiteveld J.; den Ouden J. and Sass-Klaassen U.G.W 2005: Clusters of Quercus robur and Q. petraea at the Veluwe (The Netherlands). Centre for Genetic Resources Report 1. CGN/DLO Foundation. Wageningen. 19.

Curtu A.L.; Gailing O.; Leinemann L. and Finkeldey R. 2006: Genetic Variation and Differentiation Within a Natural Community of Five Oak Species (Quercus spp.). Plant Biology 9. p. 116–126. DOI: 10.1055/s-2006-924542

Curtu A.L.; Gailing O. and Finkeldey R. 2007: Evidence for hybridization and introgression within a species-rich oak (Quercus spp.) community. BMC Evolutionary Biology 2007, 7: 218 DOI: 10.1186/1471-2148-7-218

Dow B.D.; Ashley M.V. and Hove H.F. 1995: Characterization of highly variable (GA/CT)n microsatellites in the bur oak Quercus macrocarpa. Theoretical and Applied Genetics 91: 137–141. DOI: 10.1007/BF00220870

Dow, B.D. and Ashley, M.V. 1996: Microsatellite analysis of seed dispersal and parentage of saplings in bur oak, Quercus macrocarpa. Molecular Ecology 5: 615–627 DOI: 10.1111/j.1365-294X.1996.tb00357.x

Gugerli F.; Walser J.-C.; Dounavi K.; Holderegger R.; Finkeldey R. 2007: Coincidence of Small-scale Spatial Discontinuities is Leaf Morphology and Nuclear Microsatellite Variation of Quercus petraea and Q. robur in a Mixed Forest. Annals of Botany 99: 713–722. DOI: 10.1093/aob/mcm006

Mátyás V. 1967: A tölgyek dendrológiai ismertetése in: Keresztesi B. (szerk.) 1967: A tölgyek. Akadémiai Kiadó. Budapest. 51–71.

Nei M. 1978: Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.

Oakflow Final Report 2004: OAKFLOW: Intra and interspecific gene flow in oaks as mechanisms promoting diversity and adaptive potential. EU-programme: 5. Frame Research Programme 1.1.5 Sustainable agriculture, fisheries and forestry. EU project number: QLK5-2000-00960

Peakall R. and Smouse P.E. 2006: GenAlEx 6.4: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes. 6: 288–295. DOI: 10.1111/j.1471-8286.2005.01155.x

Petit R.J.; Brewer S.; Bordács S.; Burg K.; Cheddadi R.; Coart E.; Cottrell J.; Csaikl U.M.; van Dam B.; Deans J.D.; Espinel S.; Fineschi S.; Finkeldey R.; Glaz I.; Goicoechea P.G.; Jensen J.S.; Konig A.O.; Lowe A.J.; Madsen S.F.; Mátyás G.; Munro R.C.; Popescu F.; Slade D.; Tabbener H.; de Vries S.G.M.; Ziegenhagen B.; Beaulieu JL.; Kremer A. 2002: Identification of refugia and post-glacial colonisation routes of European white oaks based on chloroplast DNA and fossil pollen evidence. Forest Ecology and Management 156: 49-74. DOI: 10.1016/s0378-1127(01)00634-x

Podani J. 1997: Bevezetés a többváltozós biológiai adatfeltárás rejtelmeibe. Scientia Kiadó. Budapest. 145.

Scotti-Saintagne, C.; Mariette, S.; Porth, I.; Goicoechea, P.G.; T. Barreneche, T.; Bodenes, C.; Burg, K. and A. Kremer, A. 2004: Genome scanning for interspecific differentiation between two closely related oak species (Quercus robur L. and Q. petraea (Matt.) Liebl.). Genetics. 168: 1615–1626. DOI: 10.1534/genetics.104.026849

Steinkellner, H.; Fluch, S.; Turetschek, E.; Lexer, C.; Steiff, R.; Kremer, A.; Burg, K and Gloessl, J. 1997: Identification and characterization of (GA/CT)n microsatellite loci from Quercus petraea. Plant Molecular Biology 33, 1093–1096. DOI: 10.1023/A:1005736722794

Streiff, R.; Ducousso, A.; Lexer, C.; Steinkellner, H.; Gloessl, J. and Kremer A. 1999: Pollen dispersal inferred from paternity analysis in a mixed oak stand of Quercus robur L. and Quercus petraea (Matt.) Liebl. Molecular Ecology 8: 831–841. DOI: 10.1046/j.1365-294X.1999.00637.x

Weir B.S. 1996: Genetic Data Analysis II. Sinauer Associates Inc. Publishers Sunderland, Massachusetts. p.166.

Weising K.; Nybom H.; Wolff K. and Kahl G. 2005: DNA Fingerprinting in Plants Principles, Methods and Applications. CRC Press, Boca Raton. p. 9, 43, 217–218.

ArcMap 9.0 Software. (2011-05-13) URL

GeneMapper 4.0 Software. (2011-05-13) URL

Quercologist. Határozóprogram a Kárpát-medence tölgyfajainak meghatározásához. (2011-05-13) URL

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Cseke, K., Bordács, S. & Borovics, A. (2011): Taxonomic and genetic study of a mixed oak stand. Bulletin of Forestry Science, 1(1): 95-105. (in Hungarian)

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Volume 1, Issue 1
Pages: 95-105

First published:
1 September 2011

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