Population genetic inventory of transdanubian Leuce poplars applying RAPD and cpDNA markers

Benke Attila; Cseke Klára; Borovics Attila

Bulletin of Forestry Science / Volume 1 / Issue 1 / Pages 83-93
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Population genetic inventory of transdanubian Leuce poplars applying RAPD and cpDNA markers

Attila Benke, Klára Cseke & Attila Borovics

Correspondence

Correspondence: Benke Attila

Postal address: H-9600 Sárvár, Várkerület 30/A.

e-mail: benke[at]ertisarvar.hu

Abstract

A population genetic study of native white poplar and european trembling aspen stands was carried out with exhaustive sampling throughout the Transdanubian region. The main goal was to make a genetic inventory in different regions and to estimate the genetic diversity within and between the analysed subpopulations. RAPD and chloroplast DNA markers were used. The RAPD and PCR-RFLP analyses were performed on 267 and 300 samples, respectively. Based on two highly polymorphic RAPD primers the subpopulation from the Belsô-Somogy region showed the highest diversity in case of both species. By the results of the applied four cpDNAmarkers the highest diversity level was found in the Kis-Balaton subpopulation of white poplars, and in the Villány subpopulation of trembling aspens. No correlation was found between the geographic and genetic (Nei’s index) distances of the populations analysed by both RAPD and cpDNA markers, respectively. The genetic variation of both genetic markers was basically derived due to the genetic diversity of populations.

Keywords: Leuce poplars, molecular genetic variability, Shannon-index, RAPD, cpDNA, Nei’s genetic distance

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Allaby, M. 2004: “Shannon-Wiener index of diversity.” a Dictionary of Ecology. 2004. Encyclopedia.com. (April 12, 2010). URL

Bartha D. és Bordács S. 1990: Elektrophoretische Untersuchungen an Weißpappel-Populationen in Ungarn. Die Holzzucht, 44: 23–25.

Bartha D. 1991: Gibt es Bodenrassen bei der Weisspappel? Allgemeine Forst Zeischrift 46: 877.

Bartha D. 1999: Phänologische und taxonomische Untersuchungen bei den einheimschen Populationen der Weisspappel (Populus alba L.). Publ. Univ. Horti. Industriaeque Alimentariae 59: 85–93.

Bartha D. 2005: Ist die Graupappel eine eigene Art? Taxonomische Untersuchungen an den Populationen der Weisspappel (Populus alba L.). Allgemeine Forst Zeitschrift/Der Wald 60: 252–254.

Bisztray Gy. 2001: Molekuláris genetikai markerek. 424. In: Velich I. (ed.): Növénygenetika. Mezőgazda Kiadó, Budapest

Bordács S. 2002: A DNS-polimorfizmus elemzéséhez alkalmazott módszerek. 53. In: Mátyás Cs. (ed.): Erdészeti – természetvédelmi genetika. Mezőgazda Kiadó, Budapest

Grivet D.; Heinze B.; Vendramin GG. and Petit RJ. 2001: Genome walking with consensus primers: application to the large single copy region of chloroplast DNA. Molecular Ecology Notes 1: 345–349. DOI: 10.1046/j.1471-8278.2001.00107.x

Hajósné Novák M. 1999: A PCR-technikán alapuló módszerek. 44–48. In: Hajósné Novák M. (ed.): Genetikai variabilitás a növénynemesítésben. Mezőgazda Kiadó, Budapest

Heinze, B. 2007: A database of PCR primers for the chloroplast genomes of higher plants. Plant Methods, 3: 4. DOI: 10.1186/1746-4811-3-4

Koltay Gy. és Kopecky F. 1954: Őshonos nyáraink leromlott öröklöttségének megjavítása. Erdészeti Kutatások 2: 65–86.

Lexer, C.; Fay, M.F.; Joseph, A.; Nica, M.S. and Heinze, B. 2005: Barrier to gene flow between two ecologically divergent Populus species, P. alba (White poplar) and P. tremula (European aspen): the role of ecology and life history in gene introgression. Molecular Ecology 14: 1045–1057. DOI: 10.1111/j.1365-294x.2005.02469.x

Lexer, C.; Buerkle, C.A.; Joseph, J.A.; Heinze, B. and Fay, M.F. 2007: Admixture in European Populus hybrid zones makes feasible the mapping of loci that contribute the reproductive isolation and trait differences. Heredity 98: 74–84. DOI: 10.1038/sj.hdy.6800898

Lexer, C.; Joseph, J.; Loo, M. Van, Barbará, T.; Heinze, B.; Bartha D.; Castiglione, S.; Fay, M. and Buerkle, C. A. 2010: Genomic Admixture Analysis in European Populus spp. Reveals Unexpected Patterns of Reproductive Isolation and Mating. Genetics 186: 699–712. DOI: 10.1534/genetics.110.118828

Mátyás Cs. 2002a: A génszintű változatosság és elemzése. 15. In: Mátyás Cs. (ed.): Erdészeti – természetvédelmi genetika. Mezőgazda Kiadó, Budapest

Mátyás Cs. 2002b: Az allélváltozatosság statisztikai elemzése. 35–40. In: Mátyás Cs. (ed.): Erdészeti – természetvédelmi genetika. Mezőgazda Kiadó, Budapest

Peakall, R. and Smouse, P.E. 2006: GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295. DOI: 10.1111/j.1471-8286.2005.01155.x

Podani J. 1997: Bevezetés a többváltozós biológiai adatfeltárás rejtelmeibe. Scientia Kiadó. Budapest p.145.

Sabatti, M.; D’Ovidio, R.; Tanzarella, O.A. és Scarascia Mugnozza, G.E. 2001: Assessment of geographic variation by RAPD markers among Italian open-pollinated progenies of Populus alba L. Genetic Resources and Crop Evolution 48(5): 423–428. DOI: 10.1023/A:1012095401517

Sánchez, N.; Grau, J. M.; Alba, N.; Manzanera, J. A. and de los Angeles Bruno, M. 2000: Genetic Characterisation of Populus tremula Regions of Origin is Spain Using RAPD Fingerprints. Silvae Genetica 49(2): 66–71.

StatSoft, Inc. 2001. STATISTICA for Windows [Computer program manual]. Tulsa, OK: StatSoft, Inc., 2300 East 14th Street, Tulsa, OK 74104, phone: (918) 749-1119, fax: (918) 749;2217, email: info@statsoft.com, URL

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Benke, A., Cseke, K. & Borovics, A. (2011): Population genetic inventory of transdanubian Leuce poplars applying RAPD and cpDNA markers. Bulletin of Forestry Science, 1(1): 83-93. (in Hungarian)

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Volume 1, Issue 1
Pages: 83-93

First published:
1 September 2011

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