Identification of poplar genotypes based on DNA fingerprinting method

Cseke Klára; Benke Attila; Borovics Attila

Bulletin of Forestry Science / Volume 1 / Issue 1 / Pages 107-114
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Klára Cseke, Attila Benke & Attila Borovics

Correspondence

Correspondence: Cseke Klára

Postal address: H-9600 Sárvár, Várkerület 30/A.

e-mail: csekekl[at]ertisarvar.hu

Abstract

A molecular genetic method was tested in order to identify poplar genotypes based on their DNA fingerprints. Applying nine microsatellite (SSR) markers the individuals were clearly distinguishable from each other. Therefore, the genetic code resulted from the analysis can be used as an identification code for each individuals. The study presented here comprises the method description by the genetic identification of 36 poplar cultivars and candidate clones. The method is proposed for variety protection and for the official control of reproductive materials as well.

Keywords: poplar cultivars, microsatellite markers, genetic identification, cultivar protection

References16

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Fossati, T.; Zapelli, I.; Bisoffi, S.; Micheletti, A.; Vietto, L.; Sala F. and Castiglione, S. 2005: Genetic relationship and clonal identity in a collection of commercially relevant cultivars assessed by AFLP and SSR. Tree Genetics & Genomes. 1: 11–20. DOI: 10.1007/s11295-004-0002-9

Gergácz J. 2000: Helyzetkép a magyar erdészeti növénynemesítésről. Magkutatás, termesztés, kereskedelem, XIV. (1): 7–11. full text

Hajósné Novák M. (szerk.) 1999: Genetikai variabilitás a növénynemesítésben. Mezőgazda Kiadó. Budapest. p. 49.

Khasa, D.P.; Nadeem, S.; Thomas, B.; Robertson, A. and Bousquet, J. 2003: Application of SSR markers for parentage analysis of Populus clones. Forest Genetics 10(4): 273–281.

Peakall, R. and Smouse, P.E. 2006: GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes. 6: 288–295. DOI: 10.1111/j.1471-8286.2005.01155.x

Podani J. 1997: Bevezetés a többváltozós biológiai adatfeltárás rejtelmeibe. Scientia Kiadó. Budapest. p. 145.

Rahman, M.H.; Dayananda, S. and Rajora, O.P. 2000: Microsatellite DNA markers in Populus tremuloides. Genome 43: 293–297.

Rajora, O.P. and Rahman, M.H. 2001: Microsatellite DNA markers and their usefulness in poplars and conservation of microsatellite DNA loci in Salicaceae. in Müller-Starck G. and Shubert R. (eds.) 2001: Genetic Response of Forest Systems to Changing Environmental Conditions. Forestry Sciences, 70: 105–115. DOI: 10.1007/978-94-015-9839-2_9

Rajora, O.P. and Rahman, M.H. 2003: Microsatellite DNA and RAPD fingerprinting, identification and genetic relationship of hybrid poplar (Populus x canadensis) cultivars. Theoretical and Applied Genetics. 106: 470–477. DOI: 10.1007/s00122-002-1082-2

Rathmacher, G.; Niggemann, M.; Wypukol, H.; Gebhardt, K.; Ziegenhagen, B. and Bialozyt, R. 2009: Allelic ladder and reference genotypes for a rigorous standardization of poplar microsatellite data. Trees. 23: 573–583. DOI: 10.1007/s00468-008-0302-z

van der Schoot, J.; Pospiskova, M.; Vosman, B. and Smulders, M.J.M. 2000: Development and characterization of microsatellite markers in black poplar (Populus nigra L.). Thoeretical Applied Genetics. 101 (1–2): 317–322. DOI: 10.1007/s001220051485

Smulders, M.J.M.; van Der Schoot, J.; Arens, P. and Vosman, B. 2001: Trinucleotide repeat microsatellite markers for black poplar (Populus nigra L.). Molecular Ecology Notes 1(3): 188–190. DOI: 10.1046/j.1471-8278.2001.00071.x

StatSoft, Inc. 2001: STATISTICA for Windows [Computer program manual]. Tulsa, OK: StatSoft, Inc., 2300 East 14th Street, Tulsa, OK 74104, phone: (918) 749-1119, fax: (918) 749–2217, email: info@statsoft.com, URL

Tuskan, G.A.; Gunter, L.E.; Yang, Z.K., Yin, T.M., Sewel, M.M. and DiFazio, S.P. 2004: Characterization of Microsatellites Revealed by Genomic Sequencing of Populus trichocarpa. Canadian Journal of Forest Research. 34(1): 85–93. DOI: 10.1139/x03-283

Weising, K.; Nybom, H.; Wolff, K. and Kahl, G. 2005: DNA Fingerprinting in Plants Principles, Methods and Applications. CRS Press. p. 42, 217.

The International Populus Genome Consortium. (2011-05-13) URL

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Cite this article as:

Cseke, K., Benke, A. & Borovics, A. (2011): Identification of poplar genotypes based on DNA fingerprinting method. Bulletin of Forestry Science, 1(1): 107-114. (in Hungarian)

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Volume 1, Issue 1
Pages: 107-114

First published:
1 September 2011

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